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Chilean Journal of Agricultural Research
Instituto de Investigaciones Agropecuarias, INIA
ISSN: 0718-5820
EISSN: 0718-5839
Vol. 76, No. 1, 2016, pp. 77-83
Bioline Code: cj16011
Full paper language: English
Document type: Research Article
Document available free of charge

Chilean Journal of Agricultural Research, Vol. 76, No. 1, 2016, pp. 77-83

 en Molecular, morphological and pathogenic characterization of six strains of Metarhizium check for this species in other resources spp. (Deuteromycotina: Hyphomycetes) for the control of Aegorhinus superciliosus (Coleoptera: Curculionidae)
Sepúlveda, Maria; Vargas, Marisol; Gerding, Macarena; Ceballos, Ricardo & Oyarzúa, Pía

Abstract

Aegorhinus superciliosus is an important pest on blueberry ( Vaccinium corymbosum check for this species in other resources L.) and other fruit trees. The use of entomopathogenic fungi as Metarhizium check for this species in other resources spp. has been evaluated for the control of this insect, but variability has been observed among different strains. The aim of this study was to characterize six promising strains of Metarhizium spp. for the control of A. superciliosus. The studied strains were QuM173c, Qu-M363, Qu-M171a, Qu-M156a, Qu-M421, and Qu-M430, all of which belonged to the Chilean Collection of Microbial Genetic Resources (ChCMGR) of the Instituto de Investigaciones Agropecuarias (INIA), Chile. Molecular characterization was made by sequencing the ITS region (Internal Transcribed Spacers, ITS-5.8S rDNA). The morphology of conidia was evaluated through scanning electron microscopy and radial colony growth was evaluated in potato dextrose agar (PDA), Sabouraud dextrose agar (SDA), agar enriched with larvae of Galleria mellonella check for this species in other resources (Lepidoptera: Pyralidae) (GA), and agar enriched with adults of A. superciliosus (AA). Pathogenicity was studied based on mortality of adults of A. superciliosus inoculated with conidia. Sequencing of the ITS-5.8S rDNA region indicates that the strains belong to the clade of M. anisopliae check for this species in other resources var. anisopliae, except for Qu-M171a, which was identified as M. anisopliae var. lepidiotum. Conidia average length for the six strains was 5.09 μm and average conidia width was 1.92 μm. Radial colony growth differences were observed between strains (p < 0.01) and between different growth media (p < 0.01). The strains exhibited the highest colony growth in the GA medium, while in the AA medium they showed the lowest (p < 0.01). Pathogenicity tests show that Qu-M430 reached a 90% mortality rate (p < 0.01). Results show that there is variability between the studied strains, which is expressed in their morphology, molecular characteristics and pathogenicity towards A. superciliosus.

Keywords
Aegorhinus superciliosus; biological control; entomopathogen; Metarhizium anisopliae; radial growth; ITS regions (Internal Transcribed Spacers)

 
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