search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Chilean Journal of Agricultural Research
Instituto de Investigaciones Agropecuarias, INIA
ISSN: 0718-5820
EISSN: 0718-5839
Vol. 76, No. 3, 2016, pp. 265-272
Bioline Code: cj16035
Full paper language: English
Document type: Research Article
Document available free of charge

Chilean Journal of Agricultural Research, Vol. 76, No. 3, 2016, pp. 265-272

 en Osmopriming improves seeds germination, growth, antioxidant responses and membrane stability during early stage of Moroccan alfalfa populations under water deficit
Mouradi, Mohammed; Bouizgaren, Abdelaziz; Farissi, Mohamed; Makoudi, Bouchra; Kabbadj, Ablaa; Very, Anne-Aliénor; Sentenac, Hervé; Qaddoury, Ahmed & Ghoulam, Cherki

Abstract

Osmopriming has a positive effect on the enhancement of seeds germination and seedlings growth, especially under stress conditions. This study investigated the effects of osmopriming with polyethylene glycol on alfalfa ( Medicago sativa check for this species in other resources L.) seeds germination and seedlings antioxidant responses under drought stress. Seeds of five Moroccan alfalfa populations and an American Moapa variety were used to investigate the effect of osmopriming on seeds germination, seedlings growth, activities of antioxidant enzymes and membrane stability under two water deficit levels (-0.45 and -0.75 MPa). Seeds were primed with polyethylene glycol (PEG6000) (-0.6 MPa) for 24 h at 25 °C. The results showed that treated seeds presented higher germination rate and growth of 8 d-old seedlings than untreated ones. Particularly, osmoprimed seeds of ‘Adis-Tata’ (Ad) and ‘Riche’ (Rc) populations presented the highest final germination percentages of 90.8% and 64%, respectively, and seedlings shoot and root lengths under both levels of water deficit. The priming treatment enhanced the activity of peroxidase (PO) and catalase (CAT) and reduced the malonyldialdehyde (MDA) content and the electrolyte leakage under water deficit. Generally, the success of germination was positively correlated to PO and CAT activities and the degree of membrane stability in drought tolerant populations. However, the positive effect of the osmopriming technique on alfalfa drought tolerance remains limited in some tested populations, and severe water stress could inhibit germination and cause damages of alfalfa seedlings.

Keywords
Catalase; electrolyte leakage; germination; malonyldialdehyde; Medicago sativa; osmopriming; peroxidase; water deficit.

 
© Copyright 2017 - Chilean Journal of Agricultural Research
Alternative site location: http://www.inia.cl

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2019, Site last up-dated on 30-Oct-2019.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil