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Chilean Journal of Agricultural Research
Instituto de Investigaciones Agropecuarias, INIA
ISSN: 0718-5820
EISSN: 0718-5839
Vol. 77, No. 3, 2017, pp. 257-265
Bioline Code: cj17032
Full paper language: English
Document type: Research Article
Document available free of charge

Chilean Journal of Agricultural Research, Vol. 77, No. 3, 2017, pp. 257-265

 en Multiple resistance to ACCase and ALS-inhibiting herbicides in Beckmannia syzigachne check for this species in other resources (Steud.) Fernald without mutations in the target enzymes
Li, Wei; Zhang, Lele; Zhao, Ning; Guo, Wenlei; Liu, Weitang; Li, Lingxu & Wang, Jinxin

Abstract

American slough grass (Beckmannia syzigachne [Steud.] Fernald) is a worldwide weed and is widely distributed in rice-wheat rotations in China. Fenoxaprop-P-ethyl and mesosulfuron-methyl are two major herbicides used to control B. syzigachne. Resistance has evolved in B. syzigachne under continuous selective pressure from herbicides. This study aimed to establish the cross-resistance pattern of a resistant population and explore the potential non-target-site based resistance mechanisms of B. syzigachne. Sequencing of target enzyme genes (acetyl coenzyme A carboxylase [ACCase] and acetolactate synthase [ALS]) revealed that there were no resistance-endowing amino acid substitutions in the resistant B. syzigachne population (RF1) compared with the sensitive population (SF1), and obtained the purified materials. Furthermore, piperonyl butoxide (PBO) and malathion showed synergistic effects with fenoxaprop-P-ethyl and mesosulfuron-methyl respectively in RF1. Therefore, we speculate that the resistance observed in B. syzigachne was related with metabolic, mostly involving the cytochrome P450 enzymes. Cross resistance patterns showed that the purified resistant B. syzigachne produce high resistance to fenoxaprop-P-ethyl and pyroxsulam; intermediate resistance to flucarbazone-sodium; low resistance to quizalofop-P-ethyl, clodinafop-propargyl, sethoxydim and mesosulfuron-methyl; sensitive to clethodim pinoxaden and isoproturon.

Keywords
Herbicide resistance; metabolic resistance; P450 inhibitors; target enzymes sequencing.

 
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