Indian Journal of Cancer
Medknow Publications on behalf of Indian Cancer Society
Vol. 42, No. 1, 2005, pp. 5-10
Bioline Code: cn05003
Full paper language: English
Document type: Research Article
Document available free of charge
Indian Journal of Cancer, Vol. 42, No. 1, 2005, pp. 5-10
© Copyright 2005 Indian Journal of Cancer.
Review Article - Tissue microarrays: Potential in the Indian subcontinent|
Venkataraman Girish, Ananthanaranayanan Vijayalakshmi
Tissue microarrays (TMAs) are a means of combining hundreds of specimens of tissue on to a single slide for analysis simultaneously. The evolution of this technology to validate the results of cDNA microarrays has impacted tremendously in accurately identifying prognostic indicators significant in determining survival demographics for patients. TMAs can be generated from archival paraffin blocks, combined with sophisticated image analysis software for reading TMA immunohistochemistry, and a staggering amount of useful information can be generated in terms of the biomarkers useful in predicting patient outcome. There is a wide range of uses for the TMA technology including profiling of specific proteins in cancerous tissues and non-cancerous tissues. Given the wide variety of tissue resources available in India, investment in a dedicated TMA facility will be of immense use in the research arena in India. This review article discusses the basics of TMA construction, design, the software available for the analysis of this technology and its relevance to Indian scientists. A potential workflow structure for setting up a TMA facility is also included.
Tissue microarrays, histopathology
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