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Indian Journal of Cancer
Medknow Publications on behalf of Indian Cancer Society
ISSN: 0019-509X
EISSN: 1998-4774
Vol. 43, No. 1, 2006, pp. 12-15
Bioline Code: cn06002
Full paper language: English
Document type: Research Article
Document available free of charge

Indian Journal of Cancer, Vol. 43, No. 1, 2006, pp. 12-15

 en Comparison of uroprotective efficacy of mesna and amifostine in Cyclophosphamide- induced hemorrhagic cystitis in rats
Kanat Ozkan, Kurt E, Yalcinkaya U, Evrensel T, Manavoglu O

Abstract

Background:Hemorrhagic cystitis (HC) is a dose limiting side effect of cyclophosphamide (CYP).
AIM: In this study, we aimed to investigate the role of amifostine in the protection of CYP-induced HC and compare its efficacy with mesna.
SETTING AND DESIGN: This animal study was conducted in the Experimental Animals Breeding and Research Center of the Medical Faculty of Uludag University.
MATERIALS AND METHODS: Male Wistar rats (150-200 g; 10 rats per group) were randomly assigned to four groups. Group I (control group) received no drugs, group II received CYP (200 mg/kg, i.p.) alone, group III received amifostine (200 mg/kg, i.p.) and CYP, and group IV received CYP and mesna (40 mg/kg, i.p.) immediately and 4 and 8 h after administration of CYP. Bladders of animals were assessed macroscopically and histologically 24 h later. Gross assessment for presence of edema and hemorrhage and histological evaluation of damage to the bladder were scored according to Gray's criteria.
STATISTICAL ANALYSIS USED: For macroscopic and microscopic data, we used statistical evaluation by Kruskal-Wallis nonparametric analysis of variance followed by the Mann-Whitney U-test.
RESULTS: All the animals in group II had evidence of HC. Significant histological damage and macroscopic changes were present in this group compared to control group ( P < 0.001). The median scores for bladder damage in group III and IV were significantly lower compared to group II ( P < 0.001). When the median scores for bladder damage of group I, III, and IV were compared, there was no significant difference among these groups.
CONCLUSION: This study demonstrated the efficacy of amifostine in prevention of cyclophosphamide-induced hemorrhagic cystitis.

Keywords
Amifostine, cyclophosphamide, hemorrhagic cystitis, mesna

 
© Copyright 2006 Indian Journal of Cancer.
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