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African Crop Science Journal
African Crop Science Society
ISSN: 1021-9730
EISSN: 1021-9730
Vol. 9, No. 3, 2001, pp. 517-525 		Bioline Code: cs01071
Full paper language: English
Document type: Research Article
Document available free of charge

African Crop Science Journal, Vol. 9, No. 3, 2001, pp. 517-525

 en Biological Characteristics Of Tomato Mild Mottle Virus A Potyvirus Isolated From Tomato And Thorn Apple In Ethiopia
Hiskias, Yaynu; Lesemann, D.E. & Vetten, H.J.

Abstract

Two isolates of the virus 246/94 and 277/94, acquired from thorn apple ( Datura stramonium check for this species in other resources ) and tomato ( Lycopersicon lycopersicum check for this species in other resources L.) Karst. Ex. Farw., syn. esculentum), respectively, were characterised biologically and serologically and compared with a local isolate of Potato virus Y (PVY) isolated from tomato and other potyviruses and isolates infecting vegetables. Both isolates of TMMV infected only 16 of 28 plant species inoculated mechanically and induced indistinguishable symptoms. The most susceptible hosts were D. metel, Datura stramonium check for this species in other resources ) and Nicotiana glutinosa check for this species in other resources L. However, these isolates differed from the PVY isolate by infecting Datura spp. check for this species in other resources and Solanum demissum check for this species in other resources L., while the PVY isolate infected Chenopodium quinoa check for this species in other resources Wild and Capsicum annuum check for this species in other resources L. Isolate 277/94 was transmitted non-persistently by the aphid, Myzus persicae Sulz. from diseased tomato to virus-free D. stramonium, D. metel L., N. glutinosa check for this species in other resources and tomato plants and from these back to virus-free test plants of each species. Purified particles of isolate 277/94 contained a single protein species with a molecular weight of 39 kDa. Furthermore, in double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) antiserum to isolate 277/94 reacted strongly with a Yemeni isolate of TMMV (Y90/7), but did not react with any other potyvirus. This clearly shows that the Yemeni and Ethiopian isolates of TMMV are similar serologically.

Keywords
Aphid transmission, host range, potyvirus, serology, tomato mild mottle virus

 
 fr
Hiskias, Yaynu; Lesemann, D.E. & Vetten, H.J.

Résumé

Datura stramonium check for this species in other resources et Nicandra physalodes check for this species in other resources Gaertn en Ethiopie. Deux formes du virus 246/94 et 277/94, ont ete obtenue respectivement partir du Datura stramonium et de tomates ( Lycopersicon lycopersicum check for this species in other resources (L.) Karst. Ex. Farw., syn. esculentum) infectees. Leurs compositions biologique et sérologique ont été identifiées et comparées avec: d'autres potyvirus, un virus présent dans les pommes de terre locales Y (PVY) et isolé à partir des tomates, et d'autres formes de virus présents dans des légumes contaminés. Les deux formes du virus TMMV ont contaminé 16 des 28 plants inoculés mécaniquement, provoquant des symptômes similaires. Les plants où le virus s'est cependant le mieux développé sont: le D. metel, le stramonium check for this species in other resources et le Nicotiana glutinosa check for this species in other resources L. Cependant, ces deux formes different du virus PVY dans la mesure où ils ont contaminé le Datura check for this species in other resources spp. et le Solanum demissum check for this species in other resources L, alors que le PVY a contaminé le Chepodium quinoa check for this species in other resources Wild et le Capsicum annuum check for this species in other resources L. Le virus 277/94 a été transmis de manière non persistante par l' Aphid, Myzus persicae Sulz. Des tomates contaminées vers du D. stramonium, D. metel L., N. glutinosa check for this species in other resources et des plants de tomate saints, puis de ces plants infectés vers d'autres plans tests saints de chaque espèce. Des particules purifiées du virus (277/94) contiennent une seule espèce de protéine avec une molécule pesont 39 kDa. De plus, une enzyme sandwich d'anti-corps immunosorbent assay (DAS-ELISA) antiserum permettant d'isoler le 277/94, a réagi fortement avec la forme Yéménite du virus TMMV (Y90/7). Ceci démontre clairement que les formes Yéménite et éthiopienne du virus TMMV sont très proches d'un point de vue sérologique.

Mots Clés
Transmission des aphides, gamme des hôtes, le virus de la tomate milde mottle

 
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