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African Crop Science Journal
African Crop Science Society
ISSN: 1021-9730
EISSN: 2072-6589
Vol. 18, No. 4, 2010, pp. 207 - 213
Bioline Code: cs10024
Full paper language: English
Document type: Research Article
Document available free of charge

African Crop Science Journal, Vol. 18, No. 4, 2010, pp. 207 - 213

 en Associated Viruses Threatening Sweetpotato Improvement and Production in Ethiopia
Adane, A.

Abstract

The level of virus contamination on sweetpotato germplasm resources in Ethiopia was determined. Sweetpotato ( Ipomea batatus check for this species in other resources ) accessions in fields at Awassa and Wondo Genet Research Stations had 85% typical virus symptoms with up to 100% incidence. The associated viruses were identified by Enzyme-linked immunosorbent assay (ELISA). Accordingly, out of 57 accessions serologically tested from Awassa, 22 and 21 were found to be infected by Sweetpotato feathery mottle virus (SPFMV) and Sweetpotato chlorotic stunt virus (SPCSV). Only one was infected by Sweetpotato virus 2 (SPV2). Similar serological analysis of 122 accessions from Wondo Genet revealed that SPFMV is the most frequent (64.7) followed by SPCSV (10.7%) and SPV2 in only two accessions. This is the first report of the occurrence of SPCSV and SPV2 in Ethiopia. There was a high virus contamination level of sweetpotato germplasm resources in Ethiopia.

Keywords
Enzyme-linked immunosorbent assay, germplasm, Ipomea batatus

 
 fr
Adane, A.

Résumé

Le niveau de contamination virale sur les ressources de germoplasme de la patate douce en Ethiope a été déterminé. Les accessions de patate douce ( Ipomea batatus check for this species in other resources ) aux champs des stations de recherche d' Awassa et dans les stations de recherche de Wondo Genet a avaient (85 %) de symptômes de virus typic avec environ 100 % d'incidence. Les virus associés étaient indentifés par l'Enzyme d'analyse immunosorbant (ELISA). En conséquence, parmi les 57 accessions d' Awassa sérologiquement testées, 22 et 21 étaient trouvées infectées par la Sweetpotato feathery mottle virus (SPFMV) et Sweetpotato chlorotic stunt virus (SPCSV), alors que le sweet potato virus 2 (SPV2) n'avait affecté qu'une seule. ). Une analyse serological similaire de 122 nouvelles accessions de Wondo Genet avaient révélé que SPFMV est le plus fréquent (64,7) suivi de SPCSV (10,7%) et SPV2 dans seulement deux accessions. Ceci est le prmier rapport sur l'occurrence de SPCSV et SPV2 en Ethiopie. Il y avait eu un niveau très élevé de contamination virale du germoplasme de patate douce en Ethiopie.

Mots Clés
Enzyme-associé d'essai immunosorbant, germoplasme, Ipomea batatus

 
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