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Strain-Typing of Potato Virus Y Isolates from Potato in Nigeria by Infectivity Tests and Elisa
Mih, A.M.; Atiri, G.I. & Rossel, H.W.
Abstract
In a survey of potato viruses in Nigeria, five isolates of potato virus Y (PVY) were obtained. Two of these, PVY-BL and PVY-BL2, produced symptoms in indicator plants that resembled those caused by PVYN strains, particularly with their ability to incite systemic veinal necrosis in Samsun tobacco. Two isolates, PVY-BK and PVY-KR, reacted positively in ELISA with polyclonal antibodies prepared against PVY-BL and also shared some pathogenic properties with PVYC and PVYO strain groups. They could, however, not be detected in ELISA using monoclonal antibodies specific for PVYC and PVYO strains, an indication that they may be strains that have not previously been described or are antigenic deviants of existing ones.
Keywords
Antibodies, ELISA, PVY strains, Solanum tuberosum
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