About Bioline  All Journals  Testimonials  Membership  News  Donations

African Crop Science Journal
African Crop Science Society
ISSN: 1021-9730
EISSN: 1021-9730
Vol. 4, No. 2, 1996, pp. 223-232
Bioline Code: cs96060
Full paper language: English
Document type: Research Article
Document available free of charge

African Crop Science Journal, Vol. 4, No. 2, 1996, pp. 223-232

 en Peroxidase activity in Coffea arabica cultivars resistant and susceptible to coffee berry disease
Gichuru, E.K.; Mwang'ombe, A.W. & Gupta, V.K.


Coffea arabica L. cultivars were inoculated with
Colletotrichum coffeanum and thereafter assayed for peroxidase
activity. Peroxidase activity of extracts obtained from
non-inoculated hypocotyls, detached and attached berries did
not reveal any difference among the resistant and susceptible
cultivars. Peroxidase activity was found to increase in all C.
arabica cultivars studied after inoculation with C. coffeanum.
The highest increases in peroxidase activity were in the
inoculated susceptible cultivars. In inoculated hypocotyls,
there was no clear difference in peroxidase activity between
the medium resistant and resistant cultivars. In inoculated
detached and attached berries the medium resistant cultivar K7
was comparable to that in the susceptible cultivars, SL 28 and
SL 34,  at specific sampling periods. Inoculated hypocotyls
and detached berries had the highest range of increase in mean
peroxidase activity (56-800%) while attached berries had a
range of only 12-76%. This study suggests that high peroxidase
activity in cultivars of C. arabica is associated with
susceptibility rather than with resistance to C. coffeanum.

Colletotrichum coffeanum, hypocotyl, coffee berries

Gichuru, E.K.; Mwang'ombe, A.W. & Gupta, V.K.


Des cultivars de Coffea arabica L. ont ete inocules de
Colletotrichum coffeanum  et ensuite leur activite au
peroxidase a ete determinee. Cette activite, determinee dans
des extraits des hypocotyles non-inocules, des cerises de
cafeier attachees et detachees, n' etait pas differente pour
les cultivars resistants et susceptibles. Apres inoculation 
de Colletotrichum coffeanum, l' activite au  peroxidase
augmentait dans tous les cultivars. La plus haute augmentation
etait observee dans les cultivars susceptibles. Dans des
hypocotyles inocules, il n' y avait pas une difference
significative d'activite de peroxidase parmi les cultivars de
resistance moyenne et les cultivars resistants. Dans les
cerises de cafeier detachees et attachees, le cultivar K7 de
moyenne resistance etait comparable aux cultivars susceptibles
SL 28 et SL 34 e des periodes specifiques d' echantillonage.
L' augmentation moyenne d'activite de peroxidase etait la plus
haute (56-800%) dans les hypocotyles inocules et les cerises
de cafeier detachees tandis que dans les cerises attachees, l'
augmentation etait de 12-76%. Cette analyse montre qu' une
activite elevee de peroxidase dans les cultivars de C. arabica
est associee avec la susceptibilite plutot qu'avec la
resistance contre C. coffeanum.

Mots Clés
Colletotrichum coffeanum, hypocotyle, cerises de cafeier

© Copyright 1996 - African Crop Science Society

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2022, Site last up-dated on 27-Jul-2022.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil