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African Crop Science Journal
African Crop Science Society
ISSN: 1021-9730
EISSN: 2072-6589
Vol. 5, No. 4, 1997, pp. 419-424
Bioline Code: cs97047
Full paper language: English
Document type: Short Communication
Document available free of charge

African Crop Science Journal, Vol. 5, No. 4, 1997, pp. 419-424

 en Eradication of taro viruses from seedlings via seed rescue culture coupled with thermotherapy
Wagih, M.E.


In order to investigate appropriate methodologies for producing virus-free taro plants, Papua New Guinea semi-wild taro (SWT), which had been grown in a field germplasm collection of 437 cultivars and exhibited high degree of field resistance to leaf blight and high susceptibility to taro viral diseases, was used as a mother source of seeds. Natural flowering was common, to varying degree, among all cultivars. All SWT plants examined produced 4-5 flower heads per plant with an average of 15,000 potentially viable seeds per head. Seeds were grown aseptically in vitro via a seed rescue culture (SRC) technique and further maintained in an insect-proof greenhouse for a period of 8 months and regularly inspected. Plants regenerated from seeds treated at 60 C for 120 min and at 55 C for 120 min remained free of all taro viral disease symptoms. Both treatments, however, significantly reduced germination from 92.16% to 42.24% and 62.28% respectively. Six other treatments, although all reduced symptoms below those of the untreated control, failed to various degrees to eradicate the symptoms. In addition to morphological and physiological variability among seedlings, the plants showed a range of susceptibility to leaf blight. The importance of using thermotherapy with the SRC technique in taro germplasm conservation, breeding and quarantine is highlighted.

Alomae and bobone viruses, Colocasia esculenta, dasheen mosaic, tissue culture, thermotherapy

Wagih, M.E.


Afin qu'on puisse mener a terme les methodologies appropriees pour cultiver les choux caraibes, les choux caraibes a croissance demi-spontanee de Papua New Guinea (swt), dont la variete avait ete cultivee sur un champ de collection du plasme germinatifet ayant aussi 437 varietes et dont la resistance est tres elevee a la brulure des feuilles et aussi la sensibilite egalement elevee aux maladies virales, la variete etait utilisee comme source principale de multiplication de semences. La floraison naturelle etait habituelle, mais aux degre variants, parmi les varietes. Toutes les varietes examinees on produit 4 a 5 boutons a fleurs par plante avec une moyenne de 15,000 graines, d'une potentialite viable par bouton a fleurs . Les semences ont ete cultivees aseptiquement in vitro en utilisant une technique de conservation de semences, qui ont ete ensuite, conservees dans les serres anti-parasitaires pour une periode de 8 mois et sous la surveillance reguliere. Les plantes regenerees des semences traitees a 60 C et 55 C respectivemment pour une duree de 120 min ont demeure saines des symptomes des maladies virales de chou caraibe. Les deux traitements, par contre, ont reduit, d'une maniere considerable, leur faculte germinative de 92.16% a 42.24% et 62.28% respectivemment. Malgre quils aient pu reduire les symptomes des maladies aux niveaux plus bas que les echantillons moins non traites, les experiences menees sur six autres traitements, n'ont pas reussi a eliminer, aux degres variants, les symptomes des maladies. En plus de la variabilite morphologique et physiologique parmi les semis, les plantes ont demontre une sensibilite variante a la maladie des brulures des feulles. L'importance d'utiliser la thermotherapie avec la technique (SRC) dans la conservation du plasmegerminatif, la selection et l'etablissement des qarantaines est mis au point.

Mots Clés
Alomae and bobone viruses, Colocasia esculenta, dasheen mosaic, tissue culture, thermotherapy

© Copyright 1997 - African Crop Science Society

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