search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


African Crop Science Journal
African Crop Science Society
ISSN: 1021-9730
EISSN: 2072-6589
Vol. 6, No. 1, 1998, pp. 69-78
Bioline Code: cs98008
Full paper language: English
Document type: Research Article
Document available free of charge

African Crop Science Journal, Vol. 6, No. 1, 1998, pp. 69-78

 fr
Shoyinka, S.A. & Thottappilly, G.

Résumé

Les champs de la citrouille (potrion) a cannelures, les parcelles experimentales et les jardins privees dans 12 etats producteurs de la citrouille au sud du Nigeria ont ete etudiees entre 1987 et 1989 en ce qui concerne les incidences relatives, la distribution et la prevalance des trois virus, notamment le potyvirus de la mosaique Telfairia (TeMV), le potyvirus de la teche verinee du poivre et la race - Y de la mosaique cucumovirus du concombre. Avec l'aide du microscope electronique, les reactions de la plante hote et l'essai de l'enzyme immunosorbante (ELISA) seul ou en combinaison, un total de 584 echantillons de feuilles provenant de 85 sites a ete teste pour evaluer la presence des trois virus susceptibles d'infecter la culture. Sur un total de 584 echantillons testes serologiquement, 498 (soit 85%) ont reagi positivement au TeMV. Toutefois, aucune reaction n'a ete obtenue lorsque les echantillons etaient testes contre l'antisera au CMV ou au PVMV. L'infestion TeMV a ete largement repandue sur la surface etudiee et son incidence variait considerablement jusqu' a 100% sur certaines surfaces. Ni PVMV ni CMV n'ont infecte la citrouille a cannelures dans les tests en serre mais les deux virus ont ete detectes dans leurs plantes-hotes respectives, le poivre et le cerfeuil, croissant ensemble avec la citrouille a cannelures aux memes endroits ou presque. Aucune evidence des infections mixtes avec les trois virus n'a ete obtenue des etudes sur ou par de nombreux hotes, a la aide d'un microscope electronique ou d'un essai serologique. Les grandes incidences et la large distribution de TeMV enregistres dans cette etude indiquent que le virus peut avoir une grande importance economique dans la production de ce legume au Nigeria.

Mots Clés
Mosaique cucumovirus du concombre, microscope electronique, Telfairia occidentalis, Hook F., serre, potyvirus de la teche veinee du poivre, detection serologique, mosaique potyvirus de Telfairia

 
 en Occurrence of Telfairia mosaic virus (temv) in fluted pumpkin in Nigeria
Shoyinka, S.A. & Thottappilly, G.

Abstract

Fluted pumpkin (Telfaira occidentalis, Hook F.) fields, experimental plots and private gardens in 12 major pumpkin producing states in southern Nigeria were surveyed between 1987 and 1989 for relative incidences, distribution and prevalence of three viruses, namely, Telfairia mosaic potyvirus (TeMV), pepper veinal mottle potyvirus (PVMV) and Y-strain of cucumber mosaic cucumovirus (CMV). Using electron microscopy, host-plant reactions and enzyme-linked immunosorbent assay (ELISA) either alone or in combination, a total of 584 leaf samples from 85 sites was tested for the presence of the three viruses reported to infect the crop. Out of a total of 584 samples tested serologically, 498 (representing 85%) reacted positively to TeMV. However, no positive reaction was obtained when samples were tested against antisera to CMV or PVMV. TeMV infection was widely distributed in the area surveyed and its incidence varied considerably reaching 100% in some areas. Neither PVMV nor CMV infected fluted pumpkin in greenhouse tests but the two viruses were detected in their respective plant-hosts, pepper and cowpeas, growing with fluted pumpkin in the same or adjacent locations. No evidence of mixed infections with the three viruses was obtained from studies by host range, electron microscopy, or serological assay. The high incidences and wide distribution of TeMV recorded in this survey indicates that the virus may be of economic importance in the production of this vegetable in Nigeria.

Keywords
Cucumber mosaic cucumovirus, electron microscopy, greenhouse bioassay, pepper veinal mottle potyvirus, serological detection, Telfairia mosaic potyvirus

 
© Copyright 1998 - African Crop Science Society

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2022, Site last up-dated on 19-Jan-2022.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil