Brassica check for this species in other resources , Cell proliferation, DNA-damage, ISSR, Somaclonal variation"/>
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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 3, No. 2, 2000, pp. 140-148
Bioline Code: ej00013
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 3, No. 2, 2000, pp. 140-148

 en Plant genomic instability detected by microsatellite-primers
Leroy, Xavier J.; Leon, Karine & Branchard, Michel

Abstract

This report describes a new application of the Inter-Simple Sequence Repeat (ISSR) technique. This technology based on the amplification of regions between microsatellites was applied to different calli from the same cauliflower mother plant. One of the tested ISSR primers, (GATA)4, generated great polymorphism. Twelve different markers were detected on polyacrylamide gels. After sequencing, one sequence showed homology with a predicated A. thaliana gene closely related to genes involved in the regulation of cell proliferation in mammalians. This marker is characterised by three microsatellites and a palindromic sequence. Possible causes of mutations in this marker are discussed and will be investigated. ISSR amplification appears as a reliable method in the determination of genetic instability at early stages in in vitro culture.

Keywords
Brassica check for this species in other resources , Cell proliferation, DNA-damage, ISSR, Somaclonal variation

 
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