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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 4, No. 1, 2001, pp. 46-51
Bioline Code: ej01006
Full paper language: English
Document type: Short Communication
Document available free of charge

Electronic Journal of Biotechnology, Vol. 4, No. 1, 2001, pp. 46-51

 en Nucleotide sequence of a genomic clone encoding a cowpea ( Vigna unguiculata check for this species in other resources L.) trypsin inhibitor
Lawrence, Paulraj Kirubakaran; Nirmala, Jayaveeramuthu & Koundal, Kripa Ram

Abstract

A protease inhibitor gene has been isolated from native cowpea ( Vigna unguiculata check for this species in other resources L.) cv. 130 from λ-ZAP II genomic library. The nucleotide sequence of this genomic clone shared 86% homology with a cowpea trypsin inhibitor f IV mRNA, and 81% with Bowman-Birk protease inhibitor genes of soybean. The isolated gene had TATA and CAT signals in the 5' upstream region. The longest open reading frame had 504 bases, encoding 167 amino acids from the predicted coding region. The active serine centers of this protein was found between 105-158 amino acid residues, with a 69 amino acids long signal peptide in its N terminal.

Keywords
cowpea genomic library, nucleotide sequencing, sequence analysis

 
© 2001 by Universidad Católica de Valparaíso -- Chile
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