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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 4, No. 2, 2001, pp. 52-58
Bioline Code: ej01011
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 4, No. 2, 2001, pp. 52-58

 en Tilapia chromosomal growth hormone gene expression accelerates growth in transgenic zebrafish ( Danio rerio check for this species in other resources )
Morales, Reynold; Herrera, María Teresa; Arenal, Amílcar; Cruz, Asterio; Hernández, Oscar; Pimentel, Rafael; Guillén, Isabel; Martínez, Rebeca & Estrada, Mario P

Abstract

Gene transfer is economically important and model fish species has produced a great impact in modern biology and biotechnology. Transgenic zebrafish ( Danio rerio check for this species in other resources ) were generated through the co-injection of a GFP-expressing plasmid and an "all fish" transgene composed by the carp β-actin promoter and the chromosomal tilapia ( Oreochromis hornorum check for this species in other resources ) growth hormone gene. The GFP expression was a good indicator of stable transformation and allowed for high efficiency selection of transgenic fish. Transgenic F1 zebrafish grew 20% faster than full sibling non-transgenic controls.

Keywords
carp, GFP, growth hormone, tilapia, transgenic, zebrafish.

 
© 2001 by Universidad Católica de Valparaíso -- Chile
Alternative site location: http://www.ejbiotechnology.info

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