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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 4, No. 2, 2001, pp. 112-115
Bioline Code: ej01016
Full paper language: English
Document type: Short Communication
Document available free of charge

Electronic Journal of Biotechnology, Vol. 4, No. 2, 2001, pp. 112-115

 en Solid-state fermentation of Bacillus thuringiensis tolworthi check for this species in other resources to control fall armyworm in maize
Capalbo, Deise Maria Fontana; Valicente, Fernando Hercule; Moraes, Iracema de Oliveira & Pelizer, Lúcia Helena

Abstract

The well-known entomopathogenic bacterium Bacillus thuringiensis check for this species in other resources (Bt) produces a spore-crystal complex which is responsible for its biocide characteristic, and the bacterium can be obtained by fermentation, either in liquid or semi-solid substrates. This paper presents a successful way to achieve solid-state fermentation of active Bt var. tolworthi (Btt) against Spodoptera frugiperda check for this species in other resources (fall armyworm) in corn. More than 109 CFU/g were obtained using humidified rice as substrate maintained in polypropylene bags. This active complex (substrate plus spore-crystal of Bt) was prepared in order to obtain 2 x 106 spores/mL; the final suspension then sprayed via tractor on corn fields. On the treated plants, mortality of neonate larvae was 100% within two days of spraying, and all larvae were found dead on leaves. During one maize crop cycle, two applications were made, and up until 70 days after emergence it was not necessary to apply any other insecticide for fall armyworm control.

Keywords
Bacillus thuringiensis check for this species in other resources , biological control, biopesticide, semi-solid fermentation, Spodoptera frugiperda check for this species in other resources

 
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