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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 4, No. 3, 2001, pp. 120-124
Bioline Code: ej01024
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 4, No. 3, 2001, pp. 120-124

 en Immobilization and stabilization of papain on chelating sepharose: a metal chelate regenerable carrier
Afaq, Sarah & Iqbal, Jawaid

Abstract

A method for immobilization of papain has been selected based on the interaction between its histidine, cysteine and tryptophan residues with the immobilized metal ion (IMI) carrier for maximum binding on a small volume of the carrier. The immobilized papain retained high activity has improved thermal stability and the carrier could be recovered from the spent bound enzyme, to be reused. Reimmobilization of papain on the regenerated matrix was equally effective with the retention of maximum enzyme activity.

Keywords
papain, immobilized metal ion (IMI) carrier, immobilization, thermal stability, regeneration of matrix

 
© 2001 by Universidad Católica de Valparaíso -- Chile
Alternative site location: http://www.ejbiotechnology.info

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