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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 4, No. 3, 2001, pp. 146-152
Bioline Code: ej01027
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 4, No. 3, 2001, pp. 146-152

 en The effect of protective ingredients on the survival of immobilized cells of Streptococcus thermophilus check for this species in other resources to air and freeze-drying
Champagne, Claude P. & Gardner, Nancy J.


Streptococcus thermophilus check for this species in other resources cultures were grown either on trehalose or lactose, immobilized in alginate beads, dipped in various protective solutions and dried by either convection air-drying (CAD) or freeze-drying (FD). Immobilized cultures dipped in the 0.1% peptone solution did not show good survival to CAD or FD, as mortality was over 99%. There was no significant difference in mortality levels, in both methods of drying, when lactose or trehalose were used as protective ingredients. The highest survival levels (50 to 98%) were with a whey-sucrose protective medium, but this was potentially related to a higher pH and solids of the solution. Mortality levels were higher in FD than CAD, and this did not appear to be related to the fact that FD cultures had lower residual moisture contents than those dried under CAD. Cells grown on lactose had slightly higher survival rates to drying than those obtained from CAD. Trehalose-positive and trehalose-negative cultures of S. thermophilus did not show different mortality patterns to CAD or FD.

compatible solutes, lactic acid bacteria, lyophilization

© 2001 by Universidad Católica de Valparaíso -- Chile
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