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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 5, No. 1, 2002, pp. 12-20
Bioline Code: ej02008
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 5, No. 1, 2002, pp. 12-20

 en Genomic variability in Vitis vinifera check for this species in other resources L. "Sangiovese" assessed by microsatellite and non-radioactive AFLP test
Vignani, Rita; Scali, Monica; Masi, Elisa & Cresti, Mauro

Abstract

Microsatellite polymorphism analysis on 25 different "Sangiovese" accessions was carried out at eight microsatellite loci (VVS2, VVS4, VVS29, VVMD3, VVMD6, VVMD7, VVMD17 and VVMD21). In order to evaluate variability within the "Sangiovese" variety and to confirm variety identification, genotype analysis, allele distribution and pedigree information were processed with a DNA-automated sequencer running AlleleLinks software.

DNA typing revealed three cases of genetic dissimilarity compared to registered "Sangiovese" clones and the divergent accessions thought to be different clones of the same variety. The divergent accessions were different from "Sangiovese" at four microsatellite loci (VVS2, VVS4, VVMD7 and VVMD21). An innovative non-radioactive modification of AFLP genome profiling confirmed the data obtained by microsatellite amplification test. These results underline the importance of biotechnological testing, such as the PCR-based DNA tests together with traditional ampelography, in Vitis vinifera check for this species in other resources L. clone and variety selection programmes to avoid misnaming and erroneous identification and to evaluate genetic relatedness and variability within populations

Keywords
clone variation, polymerase chain reaction, sequence tagged sites, variety identification

 
© 2002 by Universidad Católica de Valparaíso -- Chile
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