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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 5, No. 1, 2002, pp. 21-28
Bioline Code: ej02009
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 5, No. 1, 2002, pp. 21-28

 en Isolation and partial purification of a metabolite from a mutant strain of Bacillus check for this species in other resources sp. with antibiotic activity against plant pathogenic agents
Bernal, Giuliano; Illanes, Andrés & Ciampi, Luigi

Abstract

The use of microorganisms for biological purposes has become an effective alternative to control plant pathogens. There are many examples of formulations using bacterial or fungal strains with biocontrol applications. Among them, members of the genus Bacillus check for this species in other resources are well known antibiotic producers. However, the increased capacity of antibiotic production obtained by direct mutagenesis of wild strains, has seldom been reported in the open literature. This research refers to the mutation of the A47 Bacillus strain, a plant pathogen antagonist, in order to obtain an improved strain with enhanced capacity to synthesize metabolites with antibiotic activity. The mutant strain M40 was obtained using the mutagenic agent acridine orange. The mutant strain showed a higher antagonistic activity than the wild type A47 against the plant pathogen Botritys cinerea check for this species in other resources (grey mould), Ralstonia solanacearum check for this species in other resources (bacterial wilt) and Erwinia carotovora var. carotovora (bacterial soft rot). The final objective was to isolate the antibiotic metabolite produced by the M40 strain and to determine its chemical and antibiotic properties. The results revealed the presence of an extracellular, thermostable and methanol-soluble metabolite that absorbed light at 212nm. These characteristics are similar to those described for cyclic antibiotic lipopeptides such as iturins.

Keywords
Bacillus subtilis check for this species in other resources , biological control, iturin

 
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