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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 5, No. 3, 2002, pp. 229-232
Bioline Code: ej02036
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 5, No. 3, 2002, pp. 229-232

 en Plumbagin production by root cultures of Plumbago rosea check for this species in other resources
Panichayupakaranant, Pharkphoom & Tewtrakul, Supinya

Abstract

Root cultures of Plumbago rosea check for this species in other resources Linn. were established from young leaf explants on solid Gamborg's B5 (B5) medium supplemented with the combination of α-naphthalene acetic acid (NAA) and kinetin in the concentration ranges of 0.5-2.0 mg/l and 0.1-0.5 mg/l, respectively. The production of plumbagin, determined by TLC-densitometry was higher [0.016 ± 0.0030% dry weight (DW)] in cultured roots obtained from B5 medium supplemented with 1.0 mg/l NAA and 0.1 mg/l kinetin. Plant selection increased the plumbagin production to 0.129 ± 0.0139% DW, while variation of sucrose and nitrogen (as (NH4)2SO4) concentration in B5 media slightly increase the plumbagin synthesis to 0.023 ± 0.0017 and 0.020 ± 0.0015% DW, respectively.

Keywords
high yielding plant, medium manipulation,naphthoquinone, Plumbago indica check for this species in other resources .

 
© 2002 by Universidad Católica de Valparaíso -- Chile
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