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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 5, No. 3, 2002, pp. 243-250
Bioline Code: ej02038
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 5, No. 3, 2002, pp. 243-250

 en Choice of the adequate quantification method for recombinant human GM-CSF produced in different host systems
Fogolín, Mariela Bollati; Eberhardt, Marcos Oggero; Kratje, Ricardo & Etcheverrigaray, Marina

Abstract

Three enzyme-linked-immunosorbent assays (ELISA) were developed and compared with a bioassay to quantify the recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF). These assays were suitable to quantify the non-glycosylated rhGM-CSF present in mixtures with variable protein content, and therefore useful for determining concentrations of the cytokine in production processes. Among these assays, the competitive ELISA, developed with a MAb that recognises an epitope not involved in glycosylation, was the only one suitable to quantify the glycosylated form of rhGM-CSF produced in mammalian cell cultures.

Keywords
bioassay, ELISA, GM-CSF, monoclonal antibody, quantification

 
© 2002 by Universidad Católica de Valparaíso -- Chile
Alternative site location: http://www.ejbiotechnology.info

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