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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 5, No. 3, 2002, pp. 279-283
Bioline Code: ej02042
Full paper language: English
Document type: Note
Document available free of charge

Electronic Journal of Biotechnology, Vol. 5, No. 3, 2002, pp. 279-283

 en A laboratory scale device for microencapsulation of genetically engineered cells into alginate beads
Fiszman, Gabriel León; Karara, Armando Luis; Finocchiaro, Liliana María Elena & Glikin, Gerardo Claudio

Abstract

The microencapsulation of recombinant cells, widely used for in vitro high-density cell culture, is a novel and potentially cost-effective method of in vivo heterologous protein delivery, where the protein producing cells are immunologically protected from tissue rejection. We report here a simple, reliable and inexpensive laboratory method to generate calcium alginate microcapsules containing genetically engineered, interleukin-2 expressing, Chinese hamster ovary (CHO) cells.

Keywords
CHO cells, interleukin-2, protein delivery

 
© 2002 by Universidad Católica de Valparaíso -- Chile
Alternative site location: http://www.ejbiotechnology.info

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