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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 6, No. 2, 2003, pp. 143-147
Bioline Code: ej03017
Full paper language: English
Document type: Short Communication
Document available free of charge

Electronic Journal of Biotechnology, Vol. 6, No. 2, 2003, pp. 143-147

 en Transformation of Monascus purpureus check for this species in other resources to hygromycin B resistance with cosmid pMOcosX reduces fertility
Lakrod, Kamolnan; Chaisrisook, Chulee & Skinner, Daniel Z.

Abstract

Albino strain KB20M1 of Monascus purpureus check for this species in other resources was genetically transformed to hygromycin B resistance with cosmid pMOcosX, using biolistic bombardment. Conidia and mycelial fragments were used as the recipient material and rupture disk strengths of 6.2 and 9.3 MPa each yielded a transformed isolate from five bombardments. Southern analysis suggested a single copy of the cosmid had integrated into each of the transformants. Both of the independent transformants formed cleistothecia but ascospore formation was greatly reduced or absent, suggesting the integration and expression of the genes carried on pMOcosX interfered with fertilization and/or ascospore formation in this homothallic fungus.

Keywords
biolistic, cosmid, hygromycin, Monascus purpureus check for this species in other resources , transformation

 
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