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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 6, No. 2, 2003, pp. 161-167
Bioline Code: ej03020
Full paper language: English
Document type: Note
Document available free of charge

Electronic Journal of Biotechnology, Vol. 6, No. 2, 2003, pp. 161-167

 en Immobilized lysozyme protein on fibrous medium: Preliminary results for microfiltration applications
Assis, Odilio B. G. & Claro, Luis C.


The protein lysozyme was deposited onto a permeable support comprising chemically functionalized glass fiber. The main objective of this study was to set a stable organic net with no effect on the medium bed permeability and a preliminary test the activity of this enzyme under immobilized conditions. The film formation is followed by atomic force microscopy (AFM) surface imaging. The effect on the bacteria Escherichia coli check for this species in other resources was tested using a simple microfiltration column. The filtration results pointed around 75% removal of bacteria in the effluent when compared to the influent concentration. The removal mechanism is assumed as being essentially due biointeraction. The surface polarity characteristics of the formed film were also considered as playing an important role, suggesting an electrostatic interaction mechanism in the microorganism removal.

enzyme immobilization, packed bed column, surface analysis

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