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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 6, No. 3, 2003, pp. 198-207
Bioline Code: ej03024
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 6, No. 3, 2003, pp. 198-207

 en Enzymological characterization of pineapple extract for potential application in oak tasar ( Antheraea proylei check for this species in other resources J.) silk cocoon cooking and reeling
Singh, Laishram Rupachandra; Devi, Yengkokpam Ranjana & Devi, Sanjenbam Kunjeshwori

Abstract

Proteinases have the potential to effect partial solubilization of the proteinaceous gum sericin involved in binding the silk strands together in cocoon, an essential step in the silk cocoon cooking and reeling. Therefore, pineapple extract rich in cysteine proteinases was enzymologically characterized for its potential application in oak tasar ( Antheraea proylei check for this species in other resources J.) silk cocoon cooking and reeling. Optimum sodium carbonate concentration (9.8 mM) and optimum temperature (60°C) for the proteinase activity were determined. Though relatively thermostable, an enhanced activity loss was observed when the extract was incubated in the temperature range 70-90°C with sodium carbonate. Bulk of the activity (80-83%) remained after 1 hr of time-dependent inactivation at 60°C. The tasar cocoon extract neither caused inhibition of the activity nor enhanced its time-dependent loss by incubation at 60°C. However, it caused an enhanced time-dependent loss of the activity by incubation at 60°C with sodium carbonate. Considering these enzymological characteristics, experimental cocoon-cooking media were constituted by taking the pineapple extract with or without sodium carbonate at room temperature or 60°C. The results of the cocoon cooking and subsequent single silk filament reeling indicated for an applicability of pineapple extract as an effective agent for the oak tasar cocoon cooking and reeling.

Keywords
cysteine proteinase, pineapple fruit extract, proteinase activity, tasar silk industry.

 
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