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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 6, No. 3, 2003, pp. 254-261
Bioline Code: ej03029
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 6, No. 3, 2003, pp. 254-261

 en Root induction in radiata pine using Agrobacterium rhizogenes check for this species in other resources
Li, Mingshan & Leung, David W. M.

Abstract

Root induction using Agrobacterium rhizogenes check for this species in other resources was conducted in hypocotyl explants, intact seedlings, de-rooted seedling cuttings and adventitious shoots of radiata pine ( Pinus radiata check for this species in other resources D. Don). Use of two A. rhizogenes strains (A4T and LB9402), with or without application of IBA, can trigger root formation in different explants. Strain LBA9402 was more effective than A4T in increasing rooting percentage and root number. Addition of 4.4 uMIBA to the medium further enhanced rooting from the cultured hypocotyl segments inoculated with the two A. rhizogenes strains. Strain LBA9402+IBA induced in about 75% of the cultured hypocotyl segments to form roots. In contrast the controls failed to initiate roots on intact seedlings or cultured segments in presence or absence of IBA. Rooting of adventitious shoots from 3 year-old radiata pine was improved following inoculation with LBA9402, suggesting that this rooting treatment has potential to aid clonal propagation of radiata pine.

Keywords
Agrobacterium rhizogenes, auxin, co-cultivation, Pinus radiate, rooting.

 
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