Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
Vol. 7, No. 1, 2004, pp. 1-4
Bioline Code: ej04001
Full paper language: English
Document type: Special Article
Document available free of charge
Electronic Journal of Biotechnology, Vol. 7, No. 1, 2004, pp. 1-4
© Copyright 2004 by Universidad Católica de Valparaíso -- Chile
BIOTECHNOLOGY ISSUES FOR DEVELOPING COUNTRIES - New biotechnological applications of coconuts|
Bustamante, José O.
Coconut is a major crop of many poor nations. The present paper shows with inexpensive fluorescence microscopy of the enhanced green fluorescent protein (EGFP), that coconut water has the capability of synthesizing proteins from recombinant DNA vectors. After only 4 hrs of 100 pM application of the EGFP plasmid (pEGFP), a strong fluorescent signal was detected with an off-the-shelf, low-tech CCD webcam. Since natural coconut water is sterile, this heat-stable delivery system may be a good option for the dissemination of food supplements and pharmaceuticals in poor nations. As expensive substances could be expressed in coconut water, through bioreactor technology or micropropagation, the concepts presented here may prove invaluable to the economic and social advancement of many developing countries.
bioreactors, coconut, farming, micropropagation, nutriceuticals, recombinant, therapeuticals, transgene, transgenic
Alternative site location: http://www.ejbiotechnology.info