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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 8, No. 1, 2005, pp. 35-42
Bioline Code: ej05006
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 8, No. 1, 2005, pp. 35-42

 en Production of an extracellular keratinase from Chryseobacterium check for this species in other resources sp. growing on raw feathers
Brandelli, Adriano & Riffel, Alessandro

Abstract

The strain Chryseobacterium check for this species in other resources sp. kr6 shown to be useful for biotechnological purposes such as hydrolysis of poultry feathers and de-hairing of bovine pelts. The effect of temperature, initial pH and media composition on protease production by this keratinolytic strain was studied. The enzyme was produced between 25 and 37°C, with maximum activity and yield at 30°C. When protease production was tested in media with different initial pH, maximum activity was observed when cultivation was carried out at 30°C and initial pH ranging from 6.0 to 8.0. Higher activity was observed when feathers or feather meal were used as growth substrates, followed by soybean meal. The addition of carbohydrates or surfactants to feather broth resulted in decrease in keratinolytic activity.

Keywords
bacteria, enzyme, keratin, protease.

 
© © 2005 by Pontificia Universidad Católica de Valparaíso -- Chile
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