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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 8, No. 1, 2005, pp. 54-62
Bioline Code: ej05008
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 8, No. 1, 2005, pp. 54-62

 en Sucrose hydrolysis catalyzed by auto-immobilized invertase into intact cells of Cladosporium cladosporioides check for this species in other resources
de Almeida, Ana Cludia Santana; de Araújo, Luciares Costa; Costa, Andressa Mendes; de Abreu, César Augusto Moraes; Lima, Maria Alice Gomes de Andrade & Palha, Maria de Los Angeles Perez Fernandez


The enzyme known as invertase (E.C. - β-D-fructofuranosidase) catalyzes the sucrose hydrolysis producing an equimolar mixture of glucose and fructose named inverted sugar. The fungus Cladosporium cladosporioides check for this species in other resources has invertase as its constituent. Hence, its use as a natural immobilized support for the invertase produces interesting results for the enzyme. The present work has the objective of determining the optimum operational conditions of auto-immobilized invertase, as well as its kinetic parameters (KM and Vmax). A complete 23 factorial planning was done for the evaluation of such parameters. Temperature, pH and agitation level were the studied variables. The hydrolysis percentage was the monitored result. Batch tests in optimum conditions were done to determine the kinetic parameters. Temperature of 70°C, pH 6 and agitation of 170 rpm were the established conditions for the hydrolysis process. The auto-immobilized invertase presented a KM of 447 mM and Vmax of 2,805 mmol/min.

Cladosporium cladosporioides, factorial planning, invertase.

© © 2005 by Pontificia Universidad Católica de Valparaíso -- Chile
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