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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 8, No. 1, 2005, pp. 63-70
Bioline Code: ej05009
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 8, No. 1, 2005, pp. 63-70

 en The upstream sequence of the phycocyanin β subunit gene from Arthrospira platensis check for this species in other resources regulates expression of gfp gene in response to light intensity
Lu, Yongzhong & Zhang, Xuecheng

Abstract

In cyanobacteria, few details are known of the mechanisms through which the expression of the light-harvesting pigment c-phycocyanin is regulated. In the present study, a 419 bp upstream sequence of the phycocyanin β subunit (cpcB) gene from Arthrospira platensis check for this species in other resources FACHB341 was fused with green fluorescent protein (gfp) gene, and a heterologous reporting system was built up to investigate the influence of light intensity on the expression of gfp gene, and the regulation function of different region of the upstream sequence of cpcB gene. Results showed that the upstream sequence of cpcB gene could drive the expression of gfp gene in Synechococcus check for this species in other resources sp. strain PCC7942, and the expression was influenced by light intensity, the lower the light intensity, the higher the gfp level. Deletion analysis revealed that a light-responsive element was located in the region -276 to-218, a promoter sequence was in the region -85 to -1, and two positive cis elements were in the -419 to -276 and the -218 to -130 regions, respectively.

Keywords
cpcB gene, deletion analysis, light intensity, Synechococcus, transformation.

 
© © 2005 by Pontificia Universidad Católica de Valparaíso -- Chile
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