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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 8, No. 1, 2005, pp. 82-106
Bioline Code: ej05011
Full paper language: English
Document type: Review Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 8, No. 1, 2005, pp. 82-106

 en T-DNA insertional mutagenesis in Arabidopsis check for this species in other resources : a tool for functional genomics
Radhamony, Resmi Nath; Prasad, Anand Mohan & Srinivasan, Ramamurthy

Abstract

With the availability of complete genome sequences of several organisms, the focus has shifted from structural genomics to functional genomics, specifically in plants where the complete genomic sequences are becoming available i.e., Arabidopsis check for this species in other resources and rice. Agrobacterium mediated transformation which is exploited for transgenic technology is also being used as an effective mutagen and as a tool for functional genomics in higher plants. Besides the fact that the insertion of T-DNA element into a gene can lead to loss or gain of function, ingenious use of a variety of vectors have led to the identification of genes and regulatory elements in Arabidopsis. In this review, we highlight the progress made in the field of functional genomics of Arabidopsis using T-DNA tagging. Since this strategy has been very successfully employed in Arabidopsis and is now being extended to other plant species, we discuss the various vectors and experimental approaches employed to tag, identify and clone genes and promoter elements in Arabidopsis using T-DNA as a tool.

Keywords
Arabidopsis, functional genomics,insertional mutagenesis, promoter trapping, T-DNA tagging.

 
© © 2005 by Pontificia Universidad Católica de Valparaíso -- Chile
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