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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 8, No. 2, 2005, pp. 170-176
Bioline Code: ej05019
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 8, No. 2, 2005, pp. 170-176

 en Expression of the antibody 14D9 in Nicotiana tabacum check for this species in other resources hairy roots
Martínez, Carolina; Petruccelli, Silvana; Giulietti, Ana María & Alvarez, María Alejandra

Abstract

Nicotiana tabacum check for this species in other resources hairy roots that express the antibody 14D9 were established. The 14D9 antibody yield obtained after 20 days of culture was 5.95 μg 14D9ml-1. The addition of the reticulum endoplasmic retention sequence KDEL demonstrated a positive effect over the intracellular 14D9 amounts with a yield increase up to 20.82 μg ml-1. DMSO increased the antibody amount in the biomass from 20.00 to 64.03 μg ml-1 while PVP (at 1.5 gl-1) and gelatine (at 5.0 gl-1) increased total 14D9 amounts in the culture medium to 25 μg and 14 μg respectively.

Keywords
catalytic antibodies, in vitro cultures, molecular farming, recombinant antibodies, tobacco.

 
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