Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
Vol. 8, No. 2, 2005, pp. 204-211
Bioline Code: ej05023
Full paper language: English
Document type: Research Article
Document available free of charge
Electronic Journal of Biotechnology, Vol. 8, No. 2, 2005, pp. 204-211
© Copyright 2005 - Pontificia Universidad Católica de Valparaíso -- Chile
Wild ornamental germplasm exploration and domestication based on biotechnological approaches. In vitro colchicine treatment to obtain a new cultivar of Scoparia montevidiensis |
Escandón, Alejandro S.; Miyajima, Ikuo; Alderete, Marisol; Hagiwara, Juan Carlos; Facciuto, Gabriela; Mata, Diego & Soto, Silvina M.
The genus Scoparia is native from Argentina. The diversity of colours and shape of their flowers bestows this genus a very interesting ornamental potential. The purpose of the present study is to explore the Scoparia species germplasm by means of in vitro polyploidization in order to improve their ornamental qualities. Accessions of S. montevidiensis var. montevidiensis, S. montevidiensis var. glandulifera, S. nudicaulis, S. hasleriana and S. dulcis were collected and maintained under greenhouse conditions. The Murashige-Skoog medium, supplemented with 0.25 mg/L BAP was used for the nodal segments multiplication of the five Scoparia species. Except for S. hasleriana, the multiplication rate of the other species ranged between 10 and 12 shoots per explant. The colchicine doses tested with S. montevidiensis were: 0.0; 0.1; 0.05; 0.01 and 0.001% (24 and 48 hrs). From a total of 364 recovered plants, 4 solid tetraploid and 16 chimeras were detected. Significant differences were observed for the size of flower, leaves, and the stem diameter among the tetraploid plants and between them and the control. The tissue culture proved to be a powerful tool both to multiply the Scoparia material incorporated to our germplasm collection and to obtain new improved varieties of this beautiful genus.
chimera, flow cytometry, micropropagation, ornamental plants, tetraploid.
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