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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 8, No. 2, 2005, pp. 212-217
Bioline Code: ej05024
Full paper language: English
Document type: Short Communication
Document available free of charge

Electronic Journal of Biotechnology, Vol. 8, No. 2, 2005, pp. 212-217

 en Growth and antibacterial activity of Lentinula edodes check for this species in other resources in liquid media supplemented with agricultural wastes
Hassegawa, Regina Hiroko; Kasuya, Maria Catarina Megumi & Vanetti, Maria Cristina Dantas

Abstract

Antibacterial activity of Lentinula edodes check for this species in other resources against Bacillus subtilis check for this species in other resources was evaluated in cell-free filtrates obtained after growth in 14 different culture media. The highest B. subtilis growth inhibition was promoted by filtrates of growth media supplemented with rice bran, vermiculite or molasses. L. edodes dry mycelial biomass in liquid culture with 0.5% added rice bran was 3.2 mg/ml, after growth for 30 days at 25°C without shaking, and 4.3 mg/ml under orbital shaking (150 rpm). However, antibacterial activity, detected between 20 and 24 days of incubation of stationary cultures, was absent in filtrates of aerated cultures. Temperatures of 20-25°C enhanced both growth and antibacterial activity. Optimum pH for L. edodes mycelial growth was 3.0-3.5, while for production of antibacterial substance(s) it was 4.5. Our results indicated that incubation conditions that enhance mycelial growth are quite different from those necessary for production of antibacterial substance(s) by L. edodes.

Keywords
bacterial inhibition, bioconversion, mycelial growth, mushroom, shiitake.

 
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