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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 8, No. 2, 2005, pp. 218-225
Bioline Code: ej05025
Full paper language: English
Document type: Short Communication
Document available free of charge

Electronic Journal of Biotechnology, Vol. 8, No. 2, 2005, pp. 218-225

 en LaNe RAGE: a new tool for genomic DNA flanking sequence determination
Park, Daniel J.

Abstract

The determination of genomic DNA sequence flanking a known region is often problematic. Existing technologies depend on multiple, efficient enzyme-catalysed preparative processing steps and/or rely on relatively inefficient 'one-sided' PCR mechanisms. I demonstrate the application of a simple 'two-sided' PCR-based approach, lariat-dependent nested PCR for rapid amplification of genomic DNA ends (LaNe RAGE), applied to the mouse GAPDH and PGK1 gene flanking sequences. This demonstration offers great promise in applications such as genome walking, transposon mutagenesis mapping and DNA fingerprinting.

Keywords
cloning, DNA fingerprinting, genomic DNA walking, PCR, sequencing, transposon mutagenesis.

 
© Copyright 2005 - Pontificia Universidad Católica de Valparaíso -- Chile
Alternative site location: http://www.ejbiotechnology.info

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