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High performance enzymatic synthesis of oleyl oleate using immobilised lipase from Candida antartica 
Radzi, Salina Mat; Basri, Mahiran; Salleh, Abu Bakar; Ariff, Arbakariya; Mohammad, Rosfarizan; Rahman, Mohd. Basyaruddin Abdul & Rahman, Raja Noor Zaliha Raja Abdul
Abstract
High performance enzymatic synthesis of oleyl oleate, a liquid wax ester was carried out by lipase-catalysed esterification of oleic acid and oleyl alcohol. Various reaction parameters were optimised to obtain high yield of oleyl oleate. The optimum condition to produce oleyl oleate was reaction time; 5 min, organic solvents of log P ≥ 3.5, temperature; 40-50°C, amount of enzyme; 0.2-0.4 g and molar ratio of oleyl alcohol to oleic acid; 2:1. The operational stability of enzyme was maintained at >90% yield up to 9 cycles. Analysis of the yield of the product showed that at optimum conditions, >95% liquid wax esters were produced.
Keywords
Oleyl oleate, immobilised lipase, esterification, liquid wax ester.
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