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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 9, No. 1, 2006, pp. 51-60
Bioline Code: ej06007
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 9, No. 1, 2006, pp. 51-60

 en
De Nicolás-Santiago, Soledad; Regalado-González, Carlos; García-Almendárez, Blanca; Fernández, Francisco J.; Téllez-Jurado, Alejandro & Huerta-Ochoa, Sergio

Abstract

Mutant strains from Aspergillus niger check for this species in other resources UAM-GS1 were produced by UV radiation to increase their hemicellulolytic and cellulolytic activity production. The mutant strains showing more enzymatic activity were those labelled GS1-S059 and GS1-S067. These strains also showed the largest relationship between diameter of hydrolysis zone and colony diameter. The mutant GS1-S067 showed a colony radial extension rate and a biomass growth rate [g biomass/(cm2 h)], 1.17 times higher than that achieved by strain UAM-GS1. The high invasive capacity makes this mutant strain a promising alternative for its use in solid substrate fermentation (SSF). The morphological properties of the two mutant strains were evaluated by using scanning electron microscopy. The diameter of the sporangium of the mutant strains GS1-S059 and GS1-S067 was significantly larger (P < 0.05) than that found for the parental strain. The hypha length and diameter of the mutant strains significantly changed (P < 0.05) compared to the parental strain. A Pearson correlation analysis on hypha length, sporangium diameter, and cellulase and xylanase activities indicated that there was a strong relationship among these variables in relation to mannanase activity. Mutant strains GS1-S059 and GS1-S067 significantly increased their level of mannanase, xylanase and cellulase production, compared to the parental strain, improving their potential industrial applications.

Keywords
Aspergillus niger, mutation, mannanase production.

 
 es Physiological, morphological, and mannanase production studies on Aspergillus niger check for this species in other resources uam-gs1 mutants
De Nicolás-Santiago, Soledad; Regalado-González, Carlos; García-Almendárez, Blanca; Fernández, Francisco J.; Téllez-Jurado, Alejandro & Huerta-Ochoa, Sergio

 
© Copyright 2006 - Pontificia Universidad Católica de Valparaíso -- Chile
Alternative site location: http://www.ejbiotechnology.info

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