search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 9, No. 1, 2006, pp. 86-91
Bioline Code: ej06010
Full paper language: English
Document type: Note
Document available free of charge

Electronic Journal of Biotechnology, Vol. 9, No. 1, 2006, pp. 86-91

 es A standardized protocol for genomic DNA isolation from Terminalia arjuna check for this species in other resources for genetic diversity analysis
Sarwat, Maryam; Negi, Madan Singh; Lakshmikumaran, Malathi; Tyagi, Akhilesh Kumar; Das, Sandip & Srivastava, Prem Shankar

 
 en
Sarwat, Maryam; Negi, Madan Singh; Lakshmikumaran, Malathi; Tyagi, Akhilesh Kumar; Das, Sandip & Srivastava, Prem Shankar

Abstract

For studying genetic diversity in natural populations of Terminalia check for this species in other resources , a medicinal plant, our attempts to isolate high quality DNA using several previously reported protocols and even modifications were unsuccessful. We therefore combined CTAB based isolation, and column based purification step, to isolate DNA from Terminalia arjuna check for this species in other resources . The DNA isolated using this standardized protocol was high in quality and suitable for restriction digestion and generation of random amplification of polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP).

Keywords
DNA extraction, medicinal plants, molecular markers, Terminalia species.

 
© Copyright 2006 - Pontificia Universidad Católica de Valparaíso -- Chile
Alternative site location: http://www.ejbiotechnology.info

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 16-Oct-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil