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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 9, No. 2, 2006, pp. 171-175
Bioline Code: ej06020
Full paper language: English
Document type: Note
Document available free of charge

Electronic Journal of Biotechnology, Vol. 9, No. 2, 2006, pp. 171-175

 en Improved affinity selection using phage display technology and off-rate based selection
Yuan, Bin; Schulz, Philip; Liu, Ruitian & Sierks, Michael R.

Abstract

Flow systems such as a BIAcore biosensor can be very efficient tools to isolate high affinity antibody fragments from affinity matured phage display libraries. Here we show that using flow based selection, we can readily isolate a variant with a 35-fold higher affinity, especially with a 7 fold better off-rate, compared to the parent clone after only a single round of selection from a second generation affinity matured phage display library. The flow system represents a fast method to isolate affinity improved antibody fragments and can be particularly useful for isolating antibodies to antigens that have poor solubility, are toxic to the host cell, or prone to aggregation.

Keywords
affinity maturation, b-amyloid (Ab), dissociation rate, phage display.

 
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