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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 9, No. 3, 2006, pp. 205-209
Bioline Code: ej06026
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 9, No. 3, 2006, pp. 205-209

 en Antimicrobial effect of polyphenols from apple skins on human bacterial pathogens
Alberto, María Rosa; Canavosio, Matías Andrés Rinsdahl & de Nadra, María Cristina Manca

Abstract

Apples possess many beneficial properties for the human health related with their high content in phenolic compounds. The antimicrobial effect of these compounds from the skin of two apple varieties, Royal Gala and Granny Smith, against human pathogens was examined. The phenolic compounds were extracted with the following solvents: A, acetone: water: acetic acid; B, ethyl acetate: methanol: water and C, ethanol: water. Total phenolic, flavonoid and non-flavonoid contents were analyzed in the extracts. The antimicrobial effect was determined using the agar diffusion method. The highest inhibitory effect of both apple varieties corresponded to extract A, which contained a high phenolic content. The Granny Smith extracts with higher phenolic content presented a superior antimicrobial effect against the selected microorganisms: Escherichia coli check for this species in other resources , Escherichia coli ATCC 25922, Escherichia coli ATCC 35218, Staphylococcus aureus ATCC 25923 check for this species in other resources , Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa check for this species in other resources ATCC 27853, Enterococcus faecalis check for this species in other resources ATCC 29212 and Listeria monocytogenes check for this species in other resources . The most sensitive microorganisms were Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853and Staphylococcus aureus ATCC 29213, whereas the most resistant strains were Escherichia coli ATCC 35218 and Escherichia coli. The results obtained demonstrate a direct relationship between the phenolic content of the extracts and the antimicrobial effect.

Keywords
bacterial inhibition, Granny Smith, Royal Gala, pathogens, phenolic extracts.

 
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