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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 9, No. 3, 2006, pp. 237-240
Bioline Code: ej06032
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 9, No. 3, 2006, pp. 237-240

 en Callus culture for biomass production of milk thistle as a potential source of milk clotting peptidases
Cimino, Cecilia; Cavalli, Sandra Vairo; Spina, Francisco; Natalucci, Claudia & Priolo, Nora

Abstract

The objective of this work was the optimization of the conditions of in vitro culture for callus production of Silybum marianum (L.) Gaertn. ( Asteraceae) check for this species in other resources . Sections of cotyledons, previously disinfected by washing successively with ethanol 70°, NaClO (10% w/v) and Tween 20 (0.05% v/v) and rinsing with sterile distilled water, were used as explants. For its initial culture, B5 medium supplemented with BA and 2,4-D solidified with phytagel was used, and a 63% survival was achieved. To obtain callus, two solid media were assayed (S1 and S2) using B5 medium supplemented with growth regulators (BA and 2,4-D or NAA and BA, respectively). The calli were grown at 25°C during 45 days in darkness. Growth kinetics was studied using S1 medium obtaining a typical growth curve with an exponential phase after 14 days of incubation (rate of growth 0.005 g dry weight/ day) and stationary phase after 35 days. The rate of growth in S2 medium was slower, and rhizogenesis was observed starting on the fifth week of incubation. From these results, the best culture medium for callus production of Silybum marianum was S1 medium.

Keywords
Asteraceae, callus, rhizogenesis, Silybum marianum.

 
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