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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 9, No. 3, 2006, pp. 276-280
Bioline Code: ej06040
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 9, No. 3, 2006, pp. 276-280

 en Meiotic study of Zea mays ssp. mays check for this species in other resources (2n = 40) x Tripsacum dactyloides check for this species in other resources (2 n = 72) hybrid and its progeny
Molina, María del Carmen; García, María Dina & Chorzempa, Silvia Elena

Abstract

Maize (2n = 40) x Tripsacum dactyloides check for this species in other resources (2n = 72) F1 hybrid plants (2n = 56) were obtained by embryo rescue and induction of somatic embryogenesis/organogenesis. Hybrid plants showed Tripsacum-like phenotypes, tolerance to stresses such as NaCl salinity and low temperatures. The more frequent meiotic configurations were 28II (24%), 24II + 2IV (19%) and 26II + 1IV (12%), with an average per cell of 0.55I + 25.18II + 1.19IV. Significant differences between plants were not observed. Pollen fertility ranged from 0% to 50%. After pollination with maize or Tripsacum, 20% of F1 plants have developed viable seeds, which originated the progeny. Thirty five percent of the progeny showed 2n = 56 chromosomes and F1 like-phenotypes, which suggests they have apomictic origin. The remaining plants were fertile and they showed maize-like phenotypes and different chromosome numbers (2n = 22, 24, 26, 28 and 30), because they kept the complete maize chromosome complement and some of the Tripsacum chromosomes. Meiotic cells showed pairing between chromosomes from both parental species, which suggests the possibility of genetic recombination between them.

Keywords
FCAyF-UNLP (grant A129), FCA-UNLZ and CONICET (grant 4650).

 
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