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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 9, No. 3, 2006, pp. 303-309
Bioline Code: ej06045
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 9, No. 3, 2006, pp. 303-309

 en RAPD and freezing resistance in Eucalyptus globulus check for this species in other resources
Fernández, Marta R.; Valenzuela, Sofía A. & Balocchi, Claudio L.


Eucalyptus globulus check for this species in other resources is the second most important forest species in Chile, after Pinus radiata check for this species in other resources . The main advantages of E. globulus are its fast growth (25 m3/ha/year) and its excellent wood quality for kraft pulp production. On the negative side, its low freezing tolerance has been an obstacle for the expansion of plantations, specifically on the foothills of the Andes. The difference in the freezing resistance between clones of E. globulus has a genetic base and, therefore, it could be detected through DNA molecular markers. Fifteen clones of E. globulus, eight classified as freezing resistant and seven as freezing sensitive were analyzed using the technique of RAPD, in order to obtain molecular markers that could differentiate between freezing sensitive and resistant clones. Eighteen primers amplified reproducible bands. Three bands were only present in freezing resistant clones, two bands of 768 bp, 602 bp obtained with UBC 218 primer and one band of 248 bp obtained with UBC 237 primer. The preliminary results indicate that polymorphism can be observed with the primers employed, but it is not possible to associate the bands with the cold resistance or susceptibility in E. globulus.

Eucalyptus globulus, freezing resistance, molecular markers, RAPD

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