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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 9, No. 4, 2006, pp. 379-385
Bioline Code: ej06056
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 9, No. 4, 2006, pp. 379-385

 en Improvement of myrosinase activity of Aspergillus check for this species in other resources sp. NR4617 by chemical mutagenesis
Rakariyatham, Nuansri; Butr-Indr, Bordin; Niamsup, Hataichanoke & Shank, Lalida

Abstract

A myrosinase (thioglucoside glucohydrolase or thioglucosidase, EC 3.2.3.147) producing fungus, Aspergillus check for this species in other resources sp. NR4617, was newly isolated from decayed soil sample obtained in Thailand and was subjected to single exposure to two chemical mutagens, ethyl methanesulfonate (EMS) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Its myrosinase production was selected on low cost medium prepared from mustard seed cake ( Brassica juncea check for this species in other resources ). Studies of production and stability of the enzyme showed that EMS mutagenesis increased myrosinase activity. Aspergillus sp. NR4617E1 produced myrosinase 1.90 U ml-1 at 36 hrs of the cultivation equivalent to 171% of the enzyme production in wild-type. The stability studies revealed that myrosinase from the mutant strains retained activity similar to wild-type at 30°C. Aspergillus sp. NR4617E1 degraded 10 mM of glucosinolate completely in 36 hrs. Enhanced myrosinase production and high yields of products (allylisothiocyanate) demonstrated that this mutant could be a new found candidate for feed detoxification and industrial allylisothiocyanate production.

Keywords
allylisothiocyanate, Aspergillus sp., β-thioglucosidase, myrosinase, EMS mutagenesis, MNNG mutagenesis.

 
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