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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 9, No. 4, 2006, pp. 400-406
Bioline Code: ej06059
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 9, No. 4, 2006, pp. 400-406

 en Production and stability studies of the bioemulsifier obtained from a new strain of Candida glabrata check for this species in other resources UCP 1002
Sarubbo, Leonie Asfora; de Luna, Juliana Moura & de Campos-Takaki, Galba Maria

Abstract

Evaluation of both tenso-active and emulsifying activities indicated that a biosurfactant was produced by the newly isolated and promising strain Candida glabrata check for this species in other resources isolated from mangrove sediments. The extracellular water-soluble emulsifying agent was isolated and identified as a heteropolymer. The maximum of bioemulsifier production was observed when the strain was grown on soluble and insoluble substrates cotton seed oil plus glucose, reaching values of 10.0 g/l after 144 hrs at 200 rpm. The cell-free culture broth containing the examined agent lowered the surface tension of the medium to 31 mN/m. Stable and compact emulsions with emulsifying activity of 75% of cotton seed oil were detected. The emulsification capacity remained practically unaltered within a wide pH (2-12), temperature (4-80°C) ranges and under NaCl concentrations up to 10%.

Keywords
biosurfactant, Candida, emulsifier, fermentation, glucose, vegetal oil.

 
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