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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 9, No. 4, 2006, pp. 407-413
Bioline Code: ej06060
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 9, No. 4, 2006, pp. 407-413

 en Production of Rhodotorula glutinis check for this species in other resources : a yeast that secretes α-L-arabinofuranosidase
Martínez, Claudio; Gertosio, Cecilia; Labbe, Angelica; Pérez, Rafael & Ganga, Maria Angélica

Abstract

Rhodotorula glutinis check for this species in other resources is a yeast that secretes the enzyme α-L-arabinofuranosidase (E.C. 3.2.1.55) into the culture medium and thus has an interesting biotechnological potential. To determine improved culture conditions of this organism, different factors of the culture media were evaluated such as the use of peptone as nitrogen source, salts composition, pH and growth temperature. Likewise, beet molasses and beet cosette were tested as industrial carbon sources to induce the production of the enzyme and how they influence the yeast growth. Based on these studies a culture medium is proposed for growth of this yeast in a continuous system. By assaying different dilution rates an average specific activity for the enzyme of 82.4 U/mg of protein was obtained.

Keywords
α-L-arabinofuranosidase yeast, enzymes, wine.

 
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