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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 9, No. 5, 2006, pp. 482-493
Bioline Code: ej06067
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 9, No. 5, 2006, pp. 482-493

 en Application of response surface methodology for glucosyltransferase production and conversion of sucrose into isomaltulose using free Erwinia check for this species in other resources sp. cells
Kawaguti, Haroldo Yukio; Manrich, Eiric & Sato, Hélia Harumi

Abstract

Isomaltulose is a structural isomer of sucrose commercially used in food industries. Glucosyltransferase produced by Erwinia check for this species in other resources sp. D12 catalyses an intramolecular transglucosylation of sucrose giving isomaltulose. The Experimental design and response surface methodology were applied for the optimization of the nutrient concentration in the culture medium for the enzyme production in shaken flasks at 200 rpm and 30°C . The three variables involved in this study were sugar cane molasses, bacteriological peptone and yeast extract Prodex Lac SD ®. The statistical analysis of the results showed that, in the range studied, all the factors had a significant effect (p < 0.05) on glucosyltransferase production and the highest enzyme activity was observed in culture medium containing sugar cane molasses (160 g/L), bacteriological peptone (20 g/L) and yeast extract Prodex Lac SD® (15 g/L). Maximum glucosyltransferase activity of 29.88 U/mL was achieved in a 6.6-L fermenter using the optimized medium. Free Erwinia sp. D12 cells were used for isomaltulose production from sucrose during fifteen successive batches. The final isomaltulose concentration of 75.6% obtained in the first batch increased to 77.21% (mean value) in the other fourteen batches and the productivity of 1.1 g/L x hr was obtained in batch process.

Keywords
batch process, Erwinia sp., free cells, glucosyltransferase, isomaltulose, response surface methodology.

 
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