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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 9, No. 5, 2006, pp. 505-511
Bioline Code: ej06069
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 9, No. 5, 2006, pp. 505-511

 en Designing and validation of genus-specific primers for human gut flora study
Rekha, Rani; Rizvi, Moshahid Alam & Jaishree, Paul

Abstract

The aim of this study, was to design and validate 16S rRNA targeted oligonucleotide genus specific primers for amplifying the predominant members of gut flora using polymerase chain reaction. Primers were validated against human faecal samples. Gut flora of a normal individual was compared with that of two diseased individuals. Our observations showed that the genera Lactobacillus check for this species in other resources , Bacteroides check for this species in other resources , Peptococcus check for this species in other resources , Bifidobacterium check for this species in other resources , and E. coli were invariably present in all studied subjects however, the absence of butyrate producing bacteria Ruminococcus check for this species in other resources and Peptostreptococcus check for this species in other resources were significant. Presence of the members of the genus, Campylobacter check for this species in other resources in both the diseased samples were also unusual.

Keywords
16S rRNA based primers, anaerobic bacteria; diarrhoea patient, polymerase chain reaction.

 
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