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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 9, No. 5, 2006, pp. 522-532
Bioline Code: ej06071
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 9, No. 5, 2006, pp. 522-532

 en Evaluation of concentrated milk whey as a supplement for SF9 Spodoptera frugiperda check for this species in other resources cells in culture
Batista, Fabiana R. X.; Pereira, Carlos A.; Mendonça, Ronaldo Z. & Moraes, Angela M.

Abstract

Insect cell culture has become increasingly useful for the production of heterologous proteins as well as of baculovirus polyhedra, and several different culture media formulations can be employed for this purpose. The goal of this work was to assess the potential of lyophylized milk whey ultra filtration retentate (MWR) when associated to yeast extract (YE), glucose and Pluronic F68 (PF68) to partially replace fetal calf serum (FBS) in Spodoptera frugiperda check for this species in other resources Sf9 cells culture in Grace's medium, aiming AgMNPV baculovirus production. Batch cultivation results showed that the yeast extract and the milk whey concentrate effectively increased cell concentration to about half the level commonly verified for the Sf900II serum-free medium. A 4-fold increase in viable cell concentration was achieved when employing 5% (w/v) MWR, 8 g/L YE, 1% (v/v) of FBS and 2.7 g.l-1 of glucose, resulting in 1.6 x 107 polyhedra.ml-1 after infection with baculovirus.

Keywords
baculovirus, concentrated milk whey, Grace's medium, insect cells, media formulation, Spodoptera frugiperda Sf9.

 
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