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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 9, No. 5, 2006, pp. 541-550
Bioline Code: ej06073
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 9, No. 5, 2006, pp. 541-550

 en Genome analysis of thirteen Colombian clostridial strains by pulsed field gel electrophoresis
Ayure, Diana Milena Quilaguy; Moreno, Zulma Rocío Suárez; Gutierrez, Fabio Ancizar Aristizábal; Morales, Jose Mauricio Bernal & Castaño, Dolly Montoya

Abstract

Pulsed field gel electrophoresis was used for estimating the size of the genome and evaluating the presence of megaplasmids in 13 native Colombian solventogenic Clostridium check for this species in other resources strains. DNA preparation and purification were optimised for obtaining differentiated restriction fragments in electrophoresis. Genomic DNA was digested with ApaI, Eco52I, SmaI and XhoI enzymes. Estimated genome size for native strains ranged from 4.0 to 4.2 mega base pairs. Larger sized plasmids were detected and the presence of genes related to megaplasmid pSOL1 was determined by polymerase chain reaction. adc gene region amplification suggested that genes related to solventogenesis in native strains may be located in an extra-chromosomal element. Determining genome size provides useful information aimed at enhancing native strains' solvent production.

Keywords
Clostridium, genome size, PFGE, pSOL1.

 
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